GALM - Antibodies - The Human Protein Atlas

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GALM

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA035472

Antibody HPA035473

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA035472

HPA035473

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA035472, RRID:AB_10669906

Atlas Antibodies Cat#HPA035473, RRID:AB_2674642

VALIDATION SUMMARY

Antibody HPA035472

Antibody HPA035473

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA035472

Antibody HPA035473

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Antibody dilution

1:8

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA035472

Antibody HPA035473

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA035473 across 44 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA035472 across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human kidney shows moderate cytoplasmic and nuclear positivity in renal tubules.

Immunohistochemical staining of human fallopian tube shows moderate cytoplasmic and nuclear positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 13 cell types

RNA: detected in 37 tissues
Protein: detected in 19 cell types

Retrieval

HIER pH6

Antibody dilution

1:1500

1:150

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA035472

Antibody HPA035473

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

37.8, 24.3

37.8, 24.3, 21.5

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA035472

Antibody HPA035473

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA035472

Antibody HPA035473

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

YPGELKVWVTYTLDGGELIVNYRAQASQATPVNLTNHSYFNLAGQASPNI
NDHEVTIEADTYLPVDETLIPTGEVAPVQGTAFDLRK

PVELGKHLQDFHLNGFDHNFCLKGSKEKHFCARVHHAASGRVLEVYTTQP
GVQFYTGNFLDGTLKGKNGAVYPKHSGFCLETQNWPDAVNQPRFP

Matching transcripts

GALM-001 - ENSP00000272252 [100%]
GALM-005 - ENSP00000399473 [100%]

GALM-001 - ENSP00000272252 [100%]
GALM-003 - ENSP00000386233 [100%]
GALM-005 - ENSP00000399473 [96%]

Other gene match

ANTIGEN VIEW

GALM-001	GALM-003	GALM-005

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.