CALM2 - Antibodies - The Human Protein Atlas

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CALM2

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA044999

Antibody CAB007790

Provider

Atlas Antibodies
Sigma-Aldrich

Epitomics

Product name

HPA044999

1716-1

Host species

Rabbit

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Supernatant

Other gene match

CALM3 - ENSG00000160014 [88%]
CALML3 - ENSG00000178363 [100%]
CALM1 - ENSG00000198668 [88%]

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA044999, RRID:AB_2679177

VALIDATION SUMMARY

Antibody HPA044999

Antibody CAB007790

Immunohistochemistry

Approved

Western blot

Supported

Protein array

Supported

IMMUNOHISTOCHEMISTRY

Antibody HPA044999

Antibody CAB007790

Standard validation

Approved

Figure description

Immunohistochemical staining of human vulva/anal skin shows moderate cytoplasmic, nuclear and membranous positivity in epidermal cells.

Immunohistochemical staining of human prostate shows cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 55 cell types

RNA: detected in 37 tissues
Protein: detected in 78 cell types

Retrieval

HIER pH6

Antibody dilution

1:450

1:3000

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA044999

Antibody CAB007790

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

21.7, 20.8, 17, 16.9, 16.8, 12.9, 11.1

21.7, 20.8, 16.8, 9.4

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA044999

Antibody CAB007790

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:6000

ANTIGEN INFORMATION

Antibody HPA044999

Antibody CAB007790

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

Antigen sequence

AELRHVMTRLGEKLSDEEVDEMIRAA

Matching transcripts

CALM2-001 - ENSP00000272298 [88%]
CALM2-006 - ENSP00000411440 [88%]
CALM2-007 - ENSP00000387065 [88%]

Other gene match

CALM3 - ENSG00000160014 [88%]
CALML3 - ENSG00000178363 [100%]
CALM1 - ENSG00000198668 [88%]

ANTIGEN VIEW

CALM2-001	CALM2-006	CALM2-007

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.