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POLR2D

ANTIBODY INFORMATION
 
 

Antibody HPA034694

  

Antibody HPA046092

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA034694   HPA046092  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 14   9  

References

    

Proper citation

 Atlas Antibodies Cat#HPA034694, RRID:AB_2674284   Atlas Antibodies Cat#HPA046092, RRID:AB_10963049  

VALIDATION SUMMARY
 
 

Antibody HPA034694

  

Antibody HPA046092

  
Immunocytochemistry

Supported    
Immunohistochemistry

  Approved  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA034694

  

Antibody HPA046092

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to nuclear speckles.    

Antibody dilution

 1:65    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA034694

  

Antibody HPA046092

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human cervix, uterine shows nuclear positivity in squamous epithelial cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 58 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:50  

Literature conformity

   Partly consistent with gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA034694

  

Antibody HPA046092

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 16.3, 12.3   16.3, 12.3  

Antibody dilution

 1:250   1:100  

PROTEIN ARRAY
 
 

Antibody HPA034694

  

Antibody HPA046092

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2150  

ANTIGEN INFORMATION
 
 

Antibody HPA034694

  

Antibody HPA046092

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 67   67  

Antigen sequence

 
NRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDE
ELQQILDDIQTKRSFQY
  
NRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDE
ELQQILDDIQTKRSFQY
 

Matching transcripts

 POLR2D-001 - ENSP00000272645 [100%]
POLR2D-003 - ENSP00000387117 [100%]
  POLR2D-001 - ENSP00000272645 [100%]
POLR2D-003 - ENSP00000387117 [100%]
 

Other gene match

    
ANTIGEN VIEW
POLR2D-001
POLR2D-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.