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CSRNP1

ANTIBODY INFORMATION
 
 

Antibody HPA045207

  

Antibody HPA058463

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA045207   HPA058463  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   13  

References

    

Proper citation

   Atlas Antibodies Cat#HPA058463, RRID:AB_2683726  

VALIDATION SUMMARY
 
 

Antibody HPA045207

  

Antibody HPA058463

  
Immunocytochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA045207

  

Antibody HPA058463

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.  

Antibody dilution

 1:192   1:94  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA045207

  

Antibody HPA058463

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 63.5   63.5  

Antibody dilution

 1:250   1:310  

PROTEIN ARRAY
 
 

Antibody HPA045207

  

Antibody HPA058463

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:3750  

ANTIGEN INFORMATION
 
 

Antibody HPA045207

  

Antibody HPA058463

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 94   70  

Antigen sequence

 
REGCENPMGRVEFNQARVQTHFIHTLTRLQLEQEAESFRELEAPAQGSPP
SPGEEALVPTFPLAKPPMNNELGDNSCSSDMTDSSTASSSASGT
  
FAQEQARARHEKLRQRLKEEKLEMLQWKLSAAGVPQAEAGLPPVVDAIDD
ASVEEDLAVAVAGGRLEEVS
 

Matching transcripts

 CSRNP1-001 - ENSP00000273153 [100%]
CSRNP1-003 - ENSP00000422532 [100%]
  CSRNP1-001 - ENSP00000273153 [100%]
CSRNP1-003 - ENSP00000422532 [100%]
 

Other gene match

    
ANTIGEN VIEW
CSRNP1-001
CSRNP1-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.