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ANTIBODY INFORMATION
 
 

Antibody HPA001524

  

Antibody HPA001525

  

Antibody CAB026209

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA001524   HPA001525   MAB1184  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 2   2   6  

References

 1   1    

Proper citation

 Atlas Antibodies Cat#HPA001524, RRID:AB_1078851   Atlas Antibodies Cat#HPA001525, RRID:AB_1078849    

VALIDATION SUMMARY
 
 

Antibody HPA001524

  

Antibody HPA001525

  

Antibody CAB026209

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry


Validated

Validated
  

Validated

Validated
   Supported  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001524

  

Antibody HPA001525

  

Antibody CAB026209

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA001525.		  
Validated

Antibody staining overlaps with antibody HPA001524.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.    

Antibody dilution

 1:39   1:25    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001524

  

Antibody HPA001525

  

Antibody CAB026209

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 
   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA001525 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA001524 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human blood vessels shows distinct positivity in plasma.   Immunohistochemical staining of human kidney shows extra-cellular positivity in blood vessels and cytoplasmic positivity in cells of renal tubules.   Immunohistochemical staining of human blood vessel shows strong positivity of blood plasma.  

Expression

 RNA: detected in 3 tissues
Protein: detected in 15 cell types		   RNA: detected in 3 tissues
Protein: detected in 7 cell types		   RNA: detected in 3 tissues
Protein: detected in 23 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:150   1:600  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001524

  

Antibody HPA001525

  

Antibody CAB026209

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400610)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400610)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 39.4, 39.3   39.4, 39.3   39.4, 39.3  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001524

  

Antibody HPA001525

  

Antibody CAB026209

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA001524

  

Antibody HPA001525

  

Antibody CAB026209

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 138   146    

Antigen sequence

 
LPPSTYVEFTVSGTDCVAKEATEAAKCNLLAEKQYGFCKATLSEKLGGAE
VAVTCMVFQTQPVSSQPQPEGANEAVPTPVVDPDAPPSPPLGAPGLPPAG
SPPDSHVLLAAPPGHQLHRAHYDLRHTFMGVVSLGSPS
  
GLIYRQPNCDDPETEEAALVAIDYINQNLPWGYKHTLNQIDEVKVWPQQP
SGELFEIEIDTLETTCHVLDPTPVARCSVRQLKEHAVEGDCDFQLLKLDG
KFSVVYAKCDSSPDSAEDVRKVCQDCPLLAPLNDTRVVHAAKAALA
  

											 
																				

																		

										
											
Matching transcripts

												
																					AHSG-001 - ENSP00000393887 [100%] 
AHSG-002 - ENSP00000273784 [100%] 

											 
																						AHSG-001 - ENSP00000393887 [100%] 
AHSG-002 - ENSP00000273784 [99%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
AHSG-001
AHSG-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001524

												
										 
																				
											
											
											
Antibody HPA001525

												
										 
																				
											
											
											
Antibody CAB026209

												
										 
																		

																	

										
											
1.

												
																						Fetuin, matrix-Gla protein and osteopontin in calcification of renal allografts

	Lorenzen JM et al

	PLoS One 2012;7(12):e52039

			
											 
																							Global proteomic profiling reveals altered proteomic signature in schizophrenia serum

	Levin Y et al

	Mol Psychiatry 2010;15(11):1088-100

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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