Fields »
ABOUT
HELP
BLOG
KLHL8

ANTIBODY INFORMATION
 
 

Antibody HPA013856

  

Antibody HPA017762

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA013856   HPA017762  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   11  

References

    

Proper citation

 Atlas Antibodies Cat#HPA013856, RRID:AB_1852594   Atlas Antibodies Cat#HPA017762, RRID:AB_2669660  

VALIDATION SUMMARY
 
 

Antibody HPA013856

  

Antibody HPA017762

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA013856

  

Antibody HPA017762

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA017762.		  
Validated

Antibody staining overlaps with antibody HPA013856.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

 1:22   1:69  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA013856

  

Antibody HPA017762

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human kidney shows strong nuclear and cytoplasmic positivity in cells in tubules.   Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 36 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:125  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA013856

  

Antibody HPA017762

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 68.8, 60.3, 48   68.8, 60.3  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA013856

  

Antibody HPA017762

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA013856

  

Antibody HPA017762

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 118   132  

Antigen sequence

 
VISVEGKVYAVGGHDGNEHLGSMEMFDPLTNKWMMKASMNTKRRGIALAS
LGGPIYAIGGLDDNTCFNDVERYDIESDQWSTVAPMNTPRGGVGSVALVN
HVYAVGGNDGMASLSSVE
  
HFHPSNCLAVRAFAESHNRIDLMDMADQYACDHFTEVVECEDFVSVSPQH
LHKLLSSSDLNIENEKQVYNAAIKWLLANPQHHSKWLDETLAQVRLPLLP
VDFLMGVVAKEQIVKQNLKCRDLLDEARNYHL
 

Matching transcripts

 KLHL8-001 - ENSP00000273963 [100%]
KLHL8-002 - ENSP00000426451 [100%]
KLHL8-003 - ENSP00000408854 [100%]
KLHL8-005 - ENSP00000424131 [100%]
  KLHL8-001 - ENSP00000273963 [100%]
KLHL8-002 - ENSP00000426451 [100%]
KLHL8-005 - ENSP00000424131 [100%]
 

Other gene match

    
ANTIGEN VIEW
KLHL8-001
KLHL8-002
KLHL8-003
KLHL8-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.