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ANTIBODY INFORMATION
 
 

Antibody HPA036294

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036294  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 7  

References

  

Proper citation

 Atlas Antibodies Cat#HPA036294, RRID:AB_10671243  

VALIDATION SUMMARY
 
 

Antibody HPA036294

  
Immunohistochemistry


Validated
  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036294

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human vulva/anal skin shows strong cytoplasmic and membranous positivity in a subset of squamous epithelial cells.  

Expression

 RNA: detected in 5 tissues
Protein: detected in 16 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:50  

Literature conformity

 Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036294

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 9.2, 6.6  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA036294

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA036294

  

Antigen

 Recombinant protein fragment  

Length (aa)

 26  

Antigen sequence

 
ITYGNECHLCTESLKSNGRVQFLHDG
 

Matching transcripts

 SPINK7-001 - ENSP00000274565 [100%]
SPINK7-005 - ENSP00000427887 [100%]
 

Other gene match

  
ANTIGEN VIEW
SPINK7-001
SPINK7-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.