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DCDC2

ANTIBODY INFORMATION
 
 

Antibody HPA031582

  

Antibody HPA031583

  

Antibody HPA031584

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA031582   HPA031583   HPA031584  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 6   13   14  

References

 1      

Proper citation

 Atlas Antibodies Cat#HPA031582, RRID:AB_10600901   Atlas Antibodies Cat#HPA031583, RRID:AB_2673944   Atlas Antibodies Cat#HPA031584, RRID:AB_2673945  

VALIDATION SUMMARY
 
 

Antibody HPA031582

  

Antibody HPA031583

  

Antibody HPA031584

  
Immunocytochemistry


Validated
    

Validated
  
Immunohistochemistry

Supported   Supported    
Western blot

Supported   Supported   Supported  
Protein array

Supported   Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA031582

  

Antibody HPA031583

  

Antibody HPA031584

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA031584.		    
Validated

Antibody staining overlaps with antibody HPA031582.		  
 

Standard validation

 Supported     Supported  

     

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to cytosol.     Immunofluorescent staining of human cell line CACO-2 shows localization to cytosol.  

Antibody dilution

 1:111     1:185  

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA031582

  

Antibody HPA031583

  

Antibody HPA031584

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunohistochemical staining of human fallopian tube shows strong positivity in cilia of glandular cells.   Immunohistochemical staining of human fallopian tube shows strong positivity in cilia of glandular cells.    

Expression

 RNA: detected in 18 tissues
Protein: detected in 56 cell types		   RNA: detected in 18 tissues
Protein: detected in 34 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:400   1:1000    

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA031582

  

Antibody HPA031583

  

Antibody HPA031584

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 52.8   52.8, 25.6   52.8  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA031582

  

Antibody HPA031583

  

Antibody HPA031584

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:30000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA031582

  

Antibody HPA031583

  

Antibody HPA031584

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 86   94   85  

Antigen sequence

 
LTKLKQNVKLKNSQETIPNSDEGIFKAGAERSETRGAAEVQEDEDTQVEV
PVDQRPAEIVDEEEDGEKANKDAEQKEDFSGMNGDL
  
DAPEQVEEILDHSEQQARPARVNGGTDEENGEELQQVNNELQLVLDKERK
SQGAGSGQDEADVDPQRPPRPEVKITSPEENENNQQNKDYAAVA
  
LEGKLVESGAELENGQFYVAVGRDKFKKLPYSELLFDKSTMRRPFGQKAS
SLPPIVGSRKSKGSGNDRHSKSTVGSSDNSSPQPL
 

Matching transcripts

 DCDC2-001 - ENSP00000367715 [100%]
  DCDC2-001 - ENSP00000367715 [100%]
DCDC2-002 - ENSP00000367711 [100%]
  DCDC2-001 - ENSP00000367715 [100%]
 

Other gene match

      
ANTIGEN VIEW
DCDC2-001
DCDC2-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA031582

  

Antibody HPA031583

  

Antibody HPA031584

  

1.

 Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression
Jeruschke S et al
PLoS One 2015;10(9):e0137043
      
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.