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Antibody staining overlaps with antibody HPA026720. Antibody staining overlaps with antibody HPA019716.
Validated
Antibody staining overlaps with antibody HPA026720. Antibody staining overlaps with antibody HPA019714.
Validated
Antibody staining overlaps with antibody HPA019716. Antibody staining overlaps with antibody HPA019714.
Standard validation
Supported
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
Antibody dilution
1:20
1:100
1:100
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA019714
Antibody HPA019716
Antibody HPA019848
Antibody HPA026720
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA026720 across 45 cell lines. Pearson correlation with HPA019848 across 45 cell lines. Pearson correlation with HPA019716 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA026720 across 45 cell lines. Pearson correlation with HPA019848 across 45 cell lines. Pearson correlation with HPA019714 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA026720 across 46 cell lines. Pearson correlation with HPA019716 across 45 cell lines. Pearson correlation with HPA019714 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA019848 across 46 cell lines. Pearson correlation with HPA019716 across 45 cell lines. Pearson correlation with HPA019714 across 45 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA026720 across 71 cell types. Spearman correlation with HPA019716 across 70 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA026720 across 72 cell types. Spearman correlation with HPA019714 across 70 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA019716 across 72 cell types. Spearman correlation with HPA019714 across 71 cell types.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in granular pattern in glandular cells.
Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 74 cell types
RNA: detected in 37 tissues Protein: detected in 75 cell types
RNA: detected in 37 tissues Protein: detected in 79 cell types
RNA: detected in 37 tissues Protein: detected in 75 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:300
1:750
1:1200
1:850
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019714
Antibody HPA019716
Antibody HPA019848
Antibody HPA026720
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
35.5, 30.9, 24.6
35.5
35.5, 30.9, 24.6
35.5, 30.9
Antibody dilution
1:250
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA019714
Antibody HPA019716
Antibody HPA019848
Antibody HPA026720
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
