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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:5
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA035582
Standard validation
Supported
Figure description
Immunohistochemical staining of human pituitary gland shows strong membranous positivity in cells in anterior lobe.
Expression
RNA: detected in 18 tissues Protein: detected in 4 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035582
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
149.4, 148.9, 148
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA035582
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
