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Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoli fibrillar center.
Antibody dilution
1:73
Literature conformity
The subcellular location is supported by literature.
WESTERN BLOT
Antibody HPA048379
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
50.2, 48.2
Antibody dilution
1:360
PROTEIN ARRAY
Antibody HPA048379
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:2900
ANTIGEN INFORMATION
Antibody HPA048379
Antigen
Recombinant protein fragment
Length (aa)
52
Antigen sequence
FLSKLQHGTQQQDLNKKERRVGTLQSTKKKKESRRATESRIPVSKSQPVT
PE
