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SIGMAR1

ANTIBODY INFORMATION
 
 

Antibody HPA018002

  

Antibody HPA024071

  

Antibody CAB013470

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Zymed
  

Product name

 HPA018002   HPA024071   42-3300  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 4   5   4  

References

 2      

Proper citation

 Atlas Antibodies Cat#HPA018002, RRID:AB_1854802   Atlas Antibodies Cat#HPA024071, RRID:AB_1854803    

VALIDATION SUMMARY
 
 

Antibody HPA018002

  

Antibody HPA024071

  

Antibody CAB013470

  
Immunohistochemistry


Validated
  

Validated
   Approved  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA018002

  

Antibody HPA024071

  

Antibody CAB013470

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA024071 across 71 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA018002 across 71 cell types.		    
 

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		   RNA: detected in 37 tissues
Protein: detected in 50 cell types		   RNA: detected in 37 tissues
Protein: detected in 63 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:15   1:35   1:40  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA018002

  

Antibody HPA024071

  

Antibody CAB013470

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 25.1, 21.5   25.1, 14.9   25.1, 21.5, 14.9  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA018002

  

Antibody HPA024071

  

Antibody CAB013470

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA018002

  

Antibody HPA024071

  

Antibody CAB013470

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 54   102    

Antigen sequence

 
FVFQREEIAQLARQYAGLDHELAFSRLIVELRRLHPGHVLPDEELQWVFV
NAGG
  
HSGRYWAEISDTIISGTFHQWREGTTKSEVFYPGETVVHGPGEATAVEWG
PNTWMVEYGRGVIPSTLAFALADTVFSTQDFLTLFYTLRSYARGLRLELT
TY
  

											 
																				

																		

										
											
Matching transcripts

												
																					SIGMAR1-001 - ENSP00000277010 [100%] 
SIGMAR1-008 - ENSP00000420022 [100%] 

											 
																						SIGMAR1-001 - ENSP00000277010 [100%] 
SIGMAR1-002 - ENSP00000368170 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SIGMAR1-001
SIGMAR1-002
SIGMAR1-008
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA018002

												
										 
																				
											
											
											
Antibody HPA024071

												
										 
																				
											
											
											
Antibody CAB013470

												
										 
																		

																	

										
											
1.

												
																						The mitochondrial heme exporter FLVCR1b mediates erythroid differentiation

	Chiabrando D et al

	J Clin Invest 2012;122(12):4569-79

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Modeling tandem AAG8-MEK inhibition in melanoma cells

	Sun B et al

	Cancer Med 2014;3(3):710-8

			Application: WB				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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