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A1CF

ANTIBODY INFORMATION
 
 

Antibody HPA037779

  

Antibody HPA044079

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA037779   HPA044079  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

Proper citation

 Atlas Antibodies Cat#HPA037779, RRID:AB_10670529   Atlas Antibodies Cat#HPA044079, RRID:AB_2678800  

VALIDATION SUMMARY
 
 

Antibody HPA037779

  

Antibody HPA044079

  
Immunocytochemistry

  Supported  
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  
Western blot

Supported   Supported  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA037779

  

Antibody HPA044079

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.  

Antibody dilution

   1:182  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037779

  

Antibody HPA044079

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 35 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA044079 across 71 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA037779 across 71 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human liver shows moderate cytoplasmic and nuclear positivity in hepatocytes.   Immunohistochemical staining of human liver shows moderate cytoplasmic and nuclear positivity in hepatocytes.  

Expression

 RNA: detected in 9 tissues
Protein: detected in 16 cell types		   RNA: detected in 9 tissues
Protein: detected in 20 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:350  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA037779

  

Antibody HPA044079

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 66, 65.2, 65, 64.3   66, 65.2, 65, 64.3  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA037779

  

Antibody HPA044079

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA037779

  

Antibody HPA044079

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 77   77  

Antigen sequence

 
GQPVYQLHSAIGQDQRQLFLYKITIPALASQNPAIHPFTPPKLSAFVDEA
KTYAAEYTLQTLGIPTDGGDGTMATAA
  
REIYMNVPVGAAGVRGLGGRGYLAYTGLGRGYQVKGDKREDKLYDILPGM
ELTPMNPVTLKPQGIKLAPQILEEICQ
 

Matching transcripts

 A1CF-001 - ENSP00000363113 [100%]
A1CF-002 - ENSP00000363105 [100%]
A1CF-004 - ENSP00000363109 [100%]
A1CF-006 - ENSP00000363107 [100%]
A1CF-201 - ENSP00000282641 [100%]
A1CF-202 - ENSP00000378868 [100%]
A1CF-203 - ENSP00000378873 [100%]
  A1CF-002 - ENSP00000363105 [100%]
A1CF-202 - ENSP00000378868 [100%]
A1CF-001 - ENSP00000363113 [88%]
A1CF-004 - ENSP00000363109 [88%]
A1CF-006 - ENSP00000363107 [88%]
A1CF-201 - ENSP00000282641 [88%]
A1CF-203 - ENSP00000378873 [88%]
 

Other gene match

    
ANTIGEN VIEW
A1CF-001
A1CF-002
A1CF-004
A1CF-006
A1CF-201
A1CF-202
A1CF-203
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.