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Immunohistochemical staining of human lymph node shows strong nuclear positivity in a fraction of cells in the reaction center.
Immunohistochemical staining of human lymph node shows moderate nucleolar/ nuclear positivity in germinal center cells and non-germinal center cells.
Immunohistochemical staining of human tonsil shows strong nuclear positivity in germinal center cells and non-germinal center cells.
Immunohistochemical staining of human tonsil shows strong nuclear positivity in germinal center cells.
Expression
RNA: detected in 23 tissues Protein: detected in 38 cell types
RNA: detected in 23 tissues Protein: detected in 35 cell types
RNA: detected in 23 tissues Protein: detected in 30 cell types
RNA: detected in 23 tissues Protein: detected in 36 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:300
1:250
1:800
1:300
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA000451
Antibody HPA001164
Antibody CAB000058
Antibody CAB068198
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Figure description
Target mass (kDa)
358.7, 358.6
358.7, 358.6, 319.4
358.7, 358.6, 319.4
358.7, 358.6, 319.4
Antibody dilution
1:500
1:500
1:200
1:1000
PROTEIN ARRAY
Antibody HPA000451
Antibody HPA001164
Antibody CAB000058
Antibody CAB068198
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:1000
1:3000
ANTIGEN INFORMATION
Antibody HPA000451
Antibody HPA001164
Antibody CAB000058
Antibody CAB068198
Antigen
Recombinant protein fragment
Recombinant protein fragment
Recombinant protein
Recombinant protein
Length (aa)
151
144
Antigen sequence
PAKVEDAADSATKPENLSSKTRGSIPTDVEVLPTETEIHNEPFLTLWLTQ
VERKIQKDSLSKPEKLGTTAGQMCSGLPGLSSVDINNFGDSINESEGIPL
KRRRVSFGGHLRPELFDENLPPNTPLKRGEAPTKRKSLVMHTPPVLKKII
K
