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CYP17A1

ANTIBODY INFORMATION
 
 

Antibody HPA048533

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA048533  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 10  

References

  

Proper citation

 Atlas Antibodies Cat#HPA048533, RRID:AB_2680432  

VALIDATION SUMMARY
 
 

Antibody HPA048533

  
Immunohistochemistry

Supported  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA048533

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.  

Expression

 RNA: detected in 9 tissues
Protein: detected in 14 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:800  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA048533

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

 

Figure description

  

Target mass (kDa)

 57.4  

Antibody dilution

 1:90  

PROTEIN ARRAY
 
 

Antibody HPA048533

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:700  

ANTIGEN INFORMATION
 
 

Antibody HPA048533

  

Antigen

 Recombinant protein fragment  

Length (aa)

 141  

Antigen sequence

 
PFLPRHGHMHNNFFKLQKKYGPIYSVRMGTKTTVIVGHHQLAKEVLIKKG
KDFSGRPQMATLDIASNNRKGIAFADSGAHWQLHRRLAMATFALFKDGDQ
KLEKIICQEISTLCDMLATHNGQSIDISFPVFVAVTNVISL
 

Matching transcripts

 CYP17A1-001 - ENSP00000358903 [100%]
 

Other gene match

  
ANTIGEN VIEW
CYP17A1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.