ADAM33 - Antibodies - The Human Protein Atlas

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ADAM33

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA067152

Antibody HPA071758

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA067152

HPA071758

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA067152, RRID:AB_2685791

VALIDATION SUMMARY

Antibody HPA067152

Antibody HPA071758

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA067152

Antibody HPA071758

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & plasma membrane.

Antibody dilution

1:62

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA067152

Antibody HPA071758

Standard validation

Approved

Figure description

Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in cells in white pulp.

Expression

RNA: detected in 32 tissues
Protein: detected in 43 cell types

Retrieval

HIER pH6

Antibody dilution

1:1000

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA067152

Antibody HPA071758

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Target mass (kDa)

87.7, 84.7, 75.3

87.7, 84.7, 75.3, 15.3

Antibody dilution

1:1020

1:210

PROTEIN ARRAY

Antibody HPA067152

Antibody HPA071758

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:12250

1:2500

ANTIGEN INFORMATION

Antibody HPA067152

Antibody HPA071758

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

NSAGDAHGNCGQDSEGHFLPCAGRDALCGKLQCQGGKPSLLAPHMVPVDS
TVHLDGQEVTCRGALALPSAQLD

VLQGHIPGQPVTPHWVLDGQPWRTVSLEEPVSKPDMGLVALEAEGQEL

Matching transcripts

ADAM33-001 - ENSP00000348912 [100%]
ADAM33-005 - ENSP00000369190 [100%]
ADAM33-201 - ENSP00000322550 [100%]
ADAM33-203 - ENSP00000484600 [100%]

ADAM33-001 - ENSP00000348912 [100%]
ADAM33-005 - ENSP00000369190 [100%]
ADAM33-201 - ENSP00000322550 [100%]
ADAM33-202 - ENSP00000483343 [100%]
ADAM33-203 - ENSP00000484600 [100%]

Other gene match

ANTIGEN VIEW

ADAM33-001	ADAM33-005	ADAM33-201	ADAM33-202	ADAM33-203

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.