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Immunofluorescent staining of human cell line RT4 shows localization to cell junctions.
Antibody dilution
1:41
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA051395
Antibody HPA060740
Standard validation
Approved
Figure description
Immunohistochemical staining of human esophagus shows moderate cytoplasmic positivity in squamous epithelial cells.
Expression
RNA: detected in 35 tissues Protein: detected in 52 cell types
Retrieval
HIER pH6
Antibody dilution
1:1800
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA051395
Antibody HPA060740
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
24.5
24.5
Antibody dilution
1:200
1:300
PROTEIN ARRAY
Antibody HPA051395
Antibody HPA060740
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
