Fields »
ABOUT
HELP
BLOG
CNTN5

ANTIBODY INFORMATION
 
 

Antibody HPA039492

  

Antibody HPA041223

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039492   HPA041223  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   8  

References

    

Proper citation

 Atlas Antibodies Cat#HPA039492, RRID:AB_10795108   Atlas Antibodies Cat#HPA041223, RRID:AB_10794549  

VALIDATION SUMMARY
 
 

Antibody HPA039492

  

Antibody HPA041223

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039492

  

Antibody HPA041223

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA041223.		  
Validated

Antibody staining overlaps with antibody HPA039492.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.  

Antibody dilution

 1:116   1:26  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039492

  

Antibody HPA041223

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in leydig cells.  

Expression

 RNA: detected in 4 tissues
Protein: detected in 58 cell types		   RNA: detected in 4 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:60   1:40  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA039492

  

Antibody HPA041223

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 120.7, 118.9, 100.3   120.7, 118.9, 112.7, 112.3  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA039492

  

Antibody HPA041223

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:30000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA039492

  

Antibody HPA041223

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 88   95  

Antigen sequence

 
KSLPGLSTSYAALLRIKKSSSSSLFGSKTRPRYSSPSLGTLSASSPSWLG
AAQNYYSPINLYHSSDAFKQDESVDYGPVFVQEPDDII
  
IVVICSAEGEPSAAPTDVKATSVSVSEILVAWKHIKESLGRPQGFEVGYW
KDMEQEDTAETVKTRGNESFVILTGLEGNTLYHFTVRAYNGAGYG
 

Matching transcripts

 CNTN5-001 - ENSP00000435637 [100%]
CNTN5-003 - ENSP00000433575 [100%]
CNTN5-006 - ENSP00000436185 [100%]
CNTN5-201 - ENSP00000279463 [100%]
  CNTN5-001 - ENSP00000435637 [100%]
CNTN5-004 - ENSP00000393229 [100%]
CNTN5-006 - ENSP00000436185 [100%]
CNTN5-201 - ENSP00000279463 [100%]
CNTN5-202 - ENSP00000478120 [100%]
 

Other gene match

    
ANTIGEN VIEW
CNTN5-001
CNTN5-003
CNTN5-004
CNTN5-006
CNTN5-201
CNTN5-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.