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N6AMT2

ANTIBODY INFORMATION
 
 

Antibody HPA040417

  

Antibody HPA042543

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA040417   HPA042543  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

Proper citation

 Atlas Antibodies Cat#HPA040417, RRID:AB_2676973   Atlas Antibodies Cat#HPA042543, RRID:AB_2678045  

VALIDATION SUMMARY
 
 

Antibody HPA040417

  

Antibody HPA042543

  
Immunocytochemistry

Approved    
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040417

  

Antibody HPA042543

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.    

Antibody dilution

 1:200    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040417

  

Antibody HPA042543

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human placenta shows moderate cytoplasmic and membranous positivity in trophoblastic cells.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 54 cell types		   RNA: detected in 37 tissues
Protein: detected in 67 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1700   1:100  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA040417

  

Antibody HPA042543

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 24.5   24.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA040417

  

Antibody HPA042543

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:300000   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA040417

  

Antibody HPA042543

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 71   73  

Antigen sequence

 
MSDLEDDETPQLSAHALAALQEFYAEQKQQIEPGEDDKYNIGIIEENWQL
SQFWYSQETALQLAQEAIAAV
  
GEGGRIACVSAPSVYQKLRELCRENFSIYIFEYDKRFAMYGEEFIFYDYN
NPLDLPERIAAHSFDIVIADPPY
 

Matching transcripts

 N6AMT2-001 - ENSP00000372202 [100%]
N6AMT2-002 - ENSP00000372206 [100%]
  N6AMT2-001 - ENSP00000372202 [100%]
N6AMT2-002 - ENSP00000372206 [100%]
 

Other gene match

    
ANTIGEN VIEW
N6AMT2-001
N6AMT2-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.