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ANTIBODY INFORMATION
 
 

Antibody HPA040786

  

Antibody HPA062766

  

Antibody CAB003782

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   HUPO-liver
  

Product name

 HPA040786   HPA062766    

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 12   14   2  

References

      

Proper citation

 Atlas Antibodies Cat#HPA040786, RRID:AB_2677136   Atlas Antibodies Cat#HPA062766, RRID:AB_2684864    

VALIDATION SUMMARY
 
 

Antibody HPA040786

  

Antibody HPA062766

  

Antibody CAB003782

  
Immunocytochemistry


Validated
  

Validated
   Supported  
Immunohistochemistry

Supported    

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040786

  

Antibody HPA062766

  

Antibody CAB003782

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA062766.		  
Validated

Antibody staining overlaps with antibody HPA040786.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

 1:40   1:8   1:200  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040786

  

Antibody HPA062766

  

Antibody CAB003782

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells.     Immunohistochemical staining of human stomach shows granular cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 72 cell types		     RNA: detected in 37 tissues
Protein: detected in 66 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:1000     1:1000  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA040786

  

Antibody HPA062766

  

Antibody CAB003782

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 69, 51.2   69, 57.6, 51.2   69, 57.6, 51.2  

Antibody dilution

 1:250   1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA040786

  

Antibody HPA062766

  

Antibody CAB003782

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA040786

  

Antibody HPA062766

  

Antibody CAB003782

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Native protein  

Length (aa)

 101   53    

Antigen sequence

 
LLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGY
LAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPP
T
  
LAIGASEDKLVPADNEKGFDVASMMSVTLSCDHRVVDGAVGAQWLAEFRK
YLE
  

											 
																				

																		

										
											
Matching transcripts

												
																					DLAT-001 - ENSP00000280346 [100%] 
DLAT-005 - ENSP00000433432 [90%] 

											 
																						DLAT-001 - ENSP00000280346 [100%] 
DLAT-002 - ENSP00000376771 [100%] 
DLAT-005 - ENSP00000433432 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
DLAT-001
DLAT-002
DLAT-005
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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