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Immunohistochemical staining of human seminal vesicle shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 29 tissues Protein: detected in 38 cell types
RNA: detected in 29 tissues Protein: detected in 18 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:300
1:150
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA041118
Antibody HPA042181
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411435)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
29.6, 26.2
29.6
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA041118
Antibody HPA042181
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
