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Immunofluorescent staining of human cell line BJ shows localization to nuclear bodies & microtubules.
Antibody dilution
1:75
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA011420
Standard validation
Approved
Figure description
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Expression
RNA: detected in 33 tissues Protein: detected in 59 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011420
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403312)
Target mass (kDa)
23.8
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA011420
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
ANTIGEN INFORMATION
Antibody HPA011420
Antigen
Recombinant protein fragment
Length (aa)
102
Antigen sequence
EKKSSLLLLYKDEERLWGEAMKPLPVVSGLRSVQVPLMLNMLQESVAPAL
SCTLCSMEGDSVSSESSSPDVHLTIQEEGADDELEETSETPLNESSEGSS
EN
