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CPB1

ANTIBODY INFORMATION
 
 

Antibody HPA038069

  

Antibody HPA046340

  

Antibody CAB026158

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA038069   HPA046340   MAB2897  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 13   13   13  

References

      

Proper citation

 Atlas Antibodies Cat#HPA038069, RRID:AB_2675816   Atlas Antibodies Cat#HPA046340, RRID:AB_2679628    

VALIDATION SUMMARY
 
 

Antibody HPA038069

  

Antibody HPA046340

  

Antibody CAB026158

  
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Uncertain   Supported   Supported  
Protein array

Supported   Approved    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038069

  

Antibody HPA046340

  

Antibody CAB026158

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA046340 across 45 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA038069 across 45 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.  

Expression

 RNA: detected in 21 tissues
Protein: detected in 5 cell types		   RNA: detected in 21 tissues
Protein: detected in 4 cell types		   RNA: detected in 21 tissues
Protein: detected in 7 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:15   1:4500   1:10000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038069

  

Antibody HPA046340

  

Antibody CAB026158

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 47.4   47.4, 23.6, 11.2   47.4, 23.6, 11.2, 6.6  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA038069

  

Antibody HPA046340

  

Antibody CAB026158

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:2050    

ANTIGEN INFORMATION
 
 

Antibody HPA038069

  

Antibody HPA046340

  

Antibody CAB026158

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 88   68    

Antigen sequence

 
LQYKVLISNLRNVVEAQFDSRVRATGHSYEKYNKWETIEAWTQQVATENP
ALISRSVIGTTFEGRAIYLLKVGKAGQNKPAIFMDCGF
  
HGGEHFEGEKVFRVNVEDENHINIIRELASTTQIDFWKPDSVTQIKPHST
VDFRVKAEDTVTVENVLK
  

											 
																				

																		

										
											
Matching transcripts

												
																					CPB1-001 - ENSP00000417222 [100%] 
CPB1-201 - ENSP00000282957 [100%] 

											 
																						CPB1-001 - ENSP00000417222 [100%] 
CPB1-003 - ENSP00000417117 [100%] 
CPB1-014 - ENSP00000419427 [100%] 
CPB1-201 - ENSP00000282957 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CPB1-001
CPB1-003
CPB1-014
CPB1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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