Expression of CAST in cancer - Summary - The Human Protein Atlas

GENERAL INFORMATION
Gene name	CAST
Gene description	Calpastatin
Protein class	Cancer-related genes Disease related genes Plasma proteins Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	28
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):	Expressed in all
HPA (cell line):	Mixed
HPA (normal tissue):	Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

General cytoplasmic expression.

ANTIBODY IHC RELIABILITY
Data reliability description	Pending external verification.
Reliability score	Approved based on 3 antibodies. HPA036881 , HPA036882 , CAB009491
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PROGNOSTIC SUMMARY
Prognostic marker in stomach cancer (unfavourable), pancreatic cancer (unfavourable) and urothelial cancer (unfavourable).
Stomach cancer p<0.001 Pancreatic cancer p<0.001 Urothelial cancer p<0.001 Show all

RNA EXPRESSION OVERVIEW
TCGA dataset RNA cancer category: Expressed in all

PROTEIN EXPRESSION

STAINING SUMMARY

HPA036881	HPA036882	CAB009491

GENE INFORMATION
Gene name	CAST
Synonyms
Description	Calpastatin (HGNC Symbol)
Entrez gene summary	The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]
Chromosome	5
Cytoband	q15
Chromosome location (bp)	96525267 - 96779595
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000153113 (version 83.38)
Entrez gene	831
UniProt	P20810 (UniProt - Evidence at protein level)
neXtProt	NX_P20810
Antibodypedia	CAST antibodies

PROTEIN BROWSER

CAST-003	CAST-004	CAST-005	CAST-006	CAST-007	CAST-008	CAST-009	CAST-010	CAST-011	CAST-012	CAST-013	CAST-014	CAST-015	CAST-016	CAST-017	CAST-018	CAST-020	CAST-023	CAST-025	CAST-026	CAST-032	CAST-033	CAST-037	CAST-038	CAST-039	CAST-041	CAST-201	CAST-202

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
CAST-003 ENSP00000312523 ENST00000309190	P20810 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Plasma proteins Cancer-related genes Mutational cancer driver genes Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0004866 [endopeptidase inhibitor activity] GO:0005515 [protein binding] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0016020 [membrane] GO:0044822 [poly(A) RNA binding] GO:2000675 [negative regulation of type B pancreatic cell apoptotic process] Show all	686 aa 74.1 kDa	No	0
CAST-004 ENSP00000339914 ENST00000341926	P20810 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Plasma proteins Cancer-related genes Mutational cancer driver genes Disease related genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0004866 [endopeptidase inhibitor activity] GO:0005515 [protein binding] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0016020 [membrane] GO:0044822 [poly(A) RNA binding] GO:2000675 [negative regulation of type B pancreatic cell apoptotic process] Show all	708 aa 76.6 kDa	No	0
CAST-005 ENSP00000343421 ENST00000338252	P20810 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Plasma proteins Cancer-related genes Mutational cancer driver genes Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0004866 [endopeptidase inhibitor activity] GO:0005515 [protein binding] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0016020 [membrane] GO:0044822 [poly(A) RNA binding] GO:2000675 [negative regulation of type B pancreatic cell apoptotic process] Show all	695 aa 75.3 kDa	No	0
CAST-006 ENSP00000422957 ENST00000505143	H0Y944 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all		288 aa 30.6 kDa	No	0
CAST-007 ENSP00000422951 ENST00000511097	F8W7E0 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0005737 [cytoplasm] Show all	393 aa 41.7 kDa	No	0
CAST-008 ENSP00000425721 ENST00000508830	P20810 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Plasma proteins Cancer-related genes Mutational cancer driver genes Disease related genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0004866 [endopeptidase inhibitor activity] GO:0005515 [protein binding] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0016020 [membrane] GO:0044822 [poly(A) RNA binding] GO:2000675 [negative regulation of type B pancreatic cell apoptotic process] Show all	791 aa 84.9 kDa	No	0
CAST-009 ENSP00000396558 ENST00000421689	E9PSG1 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all		304 aa 31.9 kDa	No	0
CAST-010 ENSP00000422176 ENST00000510756	P20810 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Plasma proteins Cancer-related genes Mutational cancer driver genes Disease related genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0004866 [endopeptidase inhibitor activity] GO:0005515 [protein binding] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0016020 [membrane] GO:0044822 [poly(A) RNA binding] GO:2000675 [negative regulation of type B pancreatic cell apoptotic process] Show all	769 aa 82.4 kDa	No	0
CAST-011 ENSP00000379157 ENST00000395812	P20810 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Plasma proteins Cancer-related genes Mutational cancer driver genes Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0001889 [liver development] GO:0002020 [protease binding] GO:0004866 [endopeptidase inhibitor activity] GO:0005515 [protein binding] GO:0005634 [nucleus] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0007343 [egg activation] GO:0007420 [brain development] GO:0007520 [myoblast fusion] GO:0007568 [aging] GO:0010466 [negative regulation of peptidase activity] GO:0010859 [calcium-dependent cysteine-type endopeptidase inhibitor activity] GO:0010951 [negative regulation of endopeptidase activity] GO:0016020 [membrane] GO:0031100 [organ regeneration] GO:0043086 [negative regulation of catalytic activity] GO:0044822 [poly(A) RNA binding] GO:0045445 [myoblast differentiation] GO:0071157 [negative regulation of cell cycle arrest] GO:2000675 [negative regulation of type B pancreatic cell apoptotic process] Show all	750 aa 80.3 kDa	No	0
CAST-012 ENSP00000422677 ENST00000508608	A0A0C4DGB5 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all		754 aa 81 kDa	No	0
CAST-013 ENSP00000421130 ENST00000511049	E7EVY3 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0005737 [cytoplasm] Show all	693 aa 74.9 kDa	No	0
CAST-014 ENSP00000424160 ENST00000510500	H0Y9H6 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all		465 aa 50.5 kDa	No	0
CAST-015 ENSP00000422929 ENST00000515663	E7EQA0 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all		431 aa 47.2 kDa	No	0
CAST-016 ENSP00000422325 ENST00000510156	E7ES10 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0005737 [cytoplasm] Show all	642 aa 69.7 kDa	No	0
CAST-017 ENSP00000426946 ENST00000509903	E9PCH5 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0005737 [cytoplasm] Show all	673 aa 72.8 kDa	No	0
CAST-018 ENSP00000423638 ENST00000511782	B7Z574 [Direct mapping] Calpastatin; cDNA FLJ56123, highly similar to Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0005737 [cytoplasm] Show all	694 aa 75 kDa	No	0
CAST-020 ENSP00000425670 ENST00000504465	E9PDE4 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0005737 [cytoplasm] Show all	636 aa 69 kDa	No	0
CAST-023 ENSP00000422612 ENST00000514845	D6RBR1 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all		150 aa 15.5 kDa	No	0
CAST-025 ENSP00000427092 ENST00000506811	E7EN75 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all		156 aa 16.7 kDa	No	0
CAST-026 ENSP00000421230 ENST00000514055	D6RGF7 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all		149 aa 15.8 kDa	No	0
CAST-032 ENSP00000426422 ENST00000512620	H0YA91 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all		301 aa 31.7 kDa	No	0
CAST-033 ENSP00000422831 ENST00000508197	E7EQK6 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all		156 aa 17 kDa	No	0
CAST-037 ENSP00000425787 ENST00000508579	E7EQ12 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all		423 aa 46.1 kDa	No	0
CAST-038 ENSP00000422807 ENST00000503828	D6RAA8 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all		78 aa 8.7 kDa	No	0
CAST-039 ENSP00000423846 ENST00000509259	D6RC54 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all		91 aa 10 kDa	No	0
CAST-041 ENSP00000412374 ENST00000437034	H0Y7F0 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all		436 aa 47.7 kDa	No	0
CAST-201 ENSP00000320319 ENST00000325674	A0A0A0MR45 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all		430 aa 47 kDa	No	0
CAST-202 ENSP00000379158 ENST00000395813	P20810 [Direct mapping] Calpastatin Show all	Predicted intracellular proteins Plasma proteins Cancer-related genes Mutational cancer driver genes Disease related genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0004866 [endopeptidase inhibitor activity] GO:0005515 [protein binding] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0016020 [membrane] GO:0044822 [poly(A) RNA binding] GO:2000675 [negative regulation of type B pancreatic cell apoptotic process] Show all	708 aa 76.6 kDa	No	0