Fields »
ABOUT
HELP
BLOG
NR4A2

ANTIBODY INFORMATION
 
 

Antibody HPA000543

  

Antibody CAB010481

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA000543   sc-990  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 4   4  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA000543, RRID:AB_1854631   Santa Cruz Biotechnology Cat#sc-990, RRID:AB_2298676  

VALIDATION SUMMARY
 
 

Antibody HPA000543

  

Antibody CAB010481

  
Immunocytochemistry

Supported    
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000543

  

Antibody CAB010481

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line SiHa shows localization to nuclear speckles.    

Antibody dilution

 1:43    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000543

  

Antibody CAB010481

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human kidney shows moderate membranous and nuclear positivity in cells in tubules.   Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and glial cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 28 cell types		   RNA: detected in 36 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:140  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000543

  

Antibody CAB010481

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 66.6, 61.4, 28.3   66.6, 61.4, 59.8, 54.6, 28.3, 16.7, 12.2  

Antibody dilution

 1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA000543

  

Antibody CAB010481

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1000    

ANTIGEN INFORMATION
 
 

Antibody HPA000543

  

Antibody CAB010481

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 121    

Antigen sequence

 
VQAQYGSSPQGASPASQSYSYHSSGEYSSDFLTPEFVKFSMDLTNTEITA
TTSLPSFSTFMDNYSTGYDVKPPCLYQMPLSGQQSSIKVEDIQMHNYQQH
SHLPPQSEEMMPHSGSVYYKP
  

											 
																				

																		

										
											
Matching transcripts

												
																					NR4A2-001 - ENSP00000344479 [100%] 
NR4A2-005 - ENSP00000406808 [100%] 
NR4A2-009 - ENSP00000386993 [100%] 
NR4A2-010 - ENSP00000386747 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
NR4A2-001
NR4A2-005
NR4A2-009
NR4A2-010
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA000543

												
										 
																				
											
											
											
Antibody CAB010481

												
										 
																		

																	

										
											
1.

												
																						Wilms tumor chromatin profiles highlight stem cell properties and a renal developmental network

	Aiden AP et al

	Cell Stem Cell 2010;6(6):591-602

			
											 
																						
											 
																				

																		

										
											
2.

												
																						From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies

	Ek S et al

	Mol Cell Proteomics 2006;5(6):1072-81

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.