LPCAT1 - Antibodies - The Human Protein Atlas

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LPCAT1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA012501

Antibody HPA022268

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA012501

HPA022268

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA012501, RRID:AB_1845224

Atlas Antibodies Cat#HPA022268, RRID:AB_1845225

VALIDATION SUMMARY

Antibody HPA012501

Antibody HPA022268

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA012501

Antibody HPA022268

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & lipid droplets.

Antibody dilution

1:400

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA012501

Antibody HPA022268

Standard validation

Supported

Figure description

Immunohistochemical staining of human lung shows strong cytoplasmic positivity in alveolar cells.

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Expression

RNA: detected in 16 tissues
Protein: detected in 69 cell types

RNA: detected in 16 tissues
Protein: detected in 66 cell types

Retrieval

HIER pH6

Antibody dilution

1:50

1:750

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA012501

Antibody HPA022268

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

59.2

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA012501

Antibody HPA022268

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA012501

Antibody HPA022268

Antigen

Recombinant protein fragment

Length (aa)

108

Antigen sequence

AILTLAPHSSYFDAIPVTMTMSSIVMKAESRDIPIWGTLIQYIRPVFVSR
SDQDSRRKTVEEIKRRAQSNGKWPQIMIFPEGTCTNRTC

GVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGLKPEKLEKDL
DRYSERARMKGGEKIGIAEFAASLEVPVSDLLEDMFSLFDESGSGEVDLR
ECVVALSV

Matching transcripts

LPCAT1-002 - ENSP00000283415 [100%]

Other gene match

ANTIGEN VIEW

LPCAT1-002

RELEVANT PUBLICATIONS

Antibody HPA012501

Antibody HPA022268

Loss of lysophosphatidylcholine acyltransferase 1 leads to photoreceptor degeneration in rd11 mice
Friedman JS et al
Proc Natl Acad Sci U S A 2010;107(35):15523-8

AAV-mediated lysophosphatidylcholine acyltransferase 1 (Lpcat1) gene replacement therapy rescues retinal degeneration in rd11 mice
Dai X et al
Invest Ophthalmol Vis Sci 2014;55(3):1724-34

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.