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CHD1

ANTIBODY INFORMATION
 
 

Antibody HPA022236

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA022236  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 10  

References

  

Proper citation

 Atlas Antibodies Cat#HPA022236, RRID:AB_2670915  

VALIDATION SUMMARY
 
 

Antibody HPA022236

  
Immunocytochemistry

Supported  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA022236

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line RH-30 shows localization to nucleus & nucleoli fibrillar center.  

Antibody dilution

 1:141  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA022236

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 55 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:75  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA022236

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

 

Figure description

  

Target mass (kDa)

 196.7  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA022236

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA022236

  

Antigen

 Recombinant protein fragment  

Length (aa)

 119  

Antigen sequence

 
QESQQNSDQNSNLNPHVIRNPDVERLKENTNHDDSSRDSYSSDRHLTQYH
DHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHRDWDHYKQDSRYYSDREKH
RKLDDHRSRDHRSNLEGSL
 

Matching transcripts

 CHD1-001 - ENSP00000483667 [100%]
CHD1-201 - ENSP00000284049 [100%]
 

Other gene match

  
ANTIGEN VIEW
CHD1-001
CHD1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.