Fields »
ABOUT
HELP
BLOG
GAL3ST2

ANTIBODY INFORMATION
 
 

Antibody HPA071809

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA071809  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 14  

References

  

Proper citation

 Atlas Antibodies Cat#HPA071809, RRID:AB_2686450  

VALIDATION SUMMARY
 
 

Antibody HPA071809

  
Immunohistochemistry


Validated
  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA071809

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human appendix shows strong granular cytoplasmic positivity in grandular cells.  

Expression

 RNA: detected in 2 tissues
Protein: detected in 3 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:40  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA071809

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 46.1  

Antibody dilution

 1:80  

PROTEIN ARRAY
 
 

Antibody HPA071809

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA071809

  

Antigen

 Recombinant protein fragment  

Length (aa)

 50  

Antigen sequence

 
YAKNNMWFDFGFDPNAQCEEGYVRARIAEVERRFRLVLIAEHLDESLVLL
 

Matching transcripts

 GAL3ST2-001 - ENSP00000192314 [100%]
 

Other gene match

  
ANTIGEN VIEW
GAL3ST2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.