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ADAMTS1
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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA031498

 

Antibody HPA031499

 

Antibody CAB016394

 

Provider

Atlas Antibodies
Sigma-Aldrich
  Atlas Antibodies
Sigma-Aldrich
  Santa Cruz Biotechnology
 

Product name

HPA031498   HPA031499   sc-47727  

Host species

Rabbit   Rabbit   Mouse  

Clonality

pAb   pAb   mAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

     

Released in version

12   12   4  

References

     

Proper citation

Atlas Antibodies Cat#HPA031498, RRID:AB_2673907   Atlas Antibodies Cat#HPA031499, RRID:AB_2673908   Santa Cruz Biotechnology Cat#sc-47727, RRID:AB_626637  

VALIDATION SUMMARY

 
 

Antibody HPA031498

 

Antibody HPA031499

 

Antibody CAB016394

 
Immunocytochemistry


Validated
 

Validated
   
Immunohistochemistry

    Uncertain  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY

 
 

Antibody HPA031498

 

Antibody HPA031499

 

Antibody CAB016394

 

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA031499.
 
Validated

Antibody staining overlaps with antibody HPA031498.
   
 

Standard validation

Supported   Supported    

     

Figure description

Immunofluorescent staining of human cell line HeLa shows localization to cytosol.   Immunofluorescent staining of human cell line HeLa shows localization to cytosol.    

Antibody dilution

1:36   1:8    

Literature conformity

The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA031498

 

Antibody HPA031499

 

Antibody CAB016394

 

Standard validation

    Uncertain  

     

Figure description

    Immunohistochemical staining of human lymph node shows cytoplasmic positivity in subset of lymphoid cells.  

Expression

    RNA: detected in 36 tissues
Protein: detected in 5 cell types
 

Retrieval

    HIER pH6  

Antibody dilution

    1:10  

Literature conformity

    Partly consistent with extensive gene/protein characterization data.  

RNA consistency

    Mainly not consistent with RNA expression data.  

WESTERN BLOT

 
 

Antibody HPA031498

 

Antibody HPA031499

 

Antibody CAB016394

 

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.
  Uncertain

Analysis performed using a standard panel of samples.
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
  Uncertain

Analysis performed using a standard panel of samples.
No bands detected.
 

     

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
 

Target mass (kDa)

105.4   105.4   105.4, 22.3, 18.8, 14.6  

Antibody dilution

1:250   1:250   1:500  

PROTEIN ARRAY

 
 

Antibody HPA031498

 

Antibody HPA031499

 

Antibody CAB016394

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
  Supported

Pass with single peak corresponding to interaction only with its own antigen.
   

     

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

1:3000   1:500    

ANTIGEN INFORMATION

 
 

Antibody HPA031498

 

Antibody HPA031499

 

Antibody CAB016394

 

Antigen

Recombinant protein fragment   Recombinant protein fragment   Native protein  

Length (aa)

74   79    

Antigen sequence

SEEDEELVVPELERAPGHGTTRLRLHAFDQQLDLELRPDSSFLAPGFTLQ
NVGRKSGSETPLPETDLAHCFYSG
 
TEDEDEGTEGEDEGPQWSPQDPALQGVGQPTGTGSIRKKRFVSSHRYVET
MLVADQSMAEFHGSGLKHYLLTLFSVAAR
   

Matching transcripts

ADAMTS1-001 - ENSP00000284984 [100%]
  ADAMTS1-001 - ENSP00000284984 [99%]
   

Other gene match

     
ANTIGEN VIEW
ADAMTS1-001
 

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