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Antibody staining overlaps with antibody HPA031499.
Validated
Antibody staining overlaps with antibody HPA031498.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line HeLa shows localization to cytosol.
Immunofluorescent staining of human cell line HeLa shows localization to cytosol.
Antibody dilution
1:36
1:8
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA031498
Antibody HPA031499
Antibody CAB016394
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human lymph node shows cytoplasmic positivity in subset of lymphoid cells.
Expression
RNA: detected in 36 tissues Protein: detected in 5 cell types
Retrieval
HIER pH6
Antibody dilution
1:10
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031498
Antibody HPA031499
Antibody CAB016394
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
105.4
105.4
105.4, 22.3, 18.8, 14.6
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA031498
Antibody HPA031499
Antibody CAB016394
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.