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ACSS1

ANTIBODY INFORMATION
 
 

Antibody HPA041014

  

Antibody HPA043228

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041014   HPA043228  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

 1   1  

Proper citation

 Atlas Antibodies Cat#HPA041014, RRID:AB_2677251   Atlas Antibodies Cat#HPA043228, RRID:AB_2678372  

VALIDATION SUMMARY
 
 

Antibody HPA041014

  

Antibody HPA043228

  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Uncertain  
Protein array

Approved   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041014

  

Antibody HPA043228

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 54 cell types		   RNA: detected in 37 tissues
Protein: detected in 50 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1500   1:75  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041014

  

Antibody HPA043228

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 74.9, 62.7   74.9, 62.7, 16.1  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA041014

  

Antibody HPA043228

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:12000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA041014

  

Antibody HPA043228

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 98   98  

Antigen sequence

 
IFAGFSAESLAGRINDAKCKVVITFNQGLRGGRVVELKKIVDEAVKHCPT
VQHVLVAHRTDNKVHMGDLDVPLEQEMAKEDPVCAPESMGSEDMLFML
  
AQPGSYPALSAQAAREPAAFWGPLARDTLVWDTPYHTVWDCDFSTGKIGW
FLGGQLNVSVNCLDQHVRKSPESVALIWERDEPGTEVRITYRELLETT
 

Matching transcripts

 ACSS1-001 - ENSP00000316924 [100%]
ACSS1-201 - ENSP00000388793 [100%]
ACSS1-202 - ENSP00000439304 [100%]
  ACSS1-001 - ENSP00000316924 [100%]
ACSS1-201 - ENSP00000388793 [100%]
ACSS1-002 - ENSP00000365916 [94%]
 

Other gene match

    
ANTIGEN VIEW
ACSS1-001
ACSS1-002
ACSS1-201
ACSS1-202

RELEVANT PUBLICATIONS
 
 

Antibody HPA041014

  

Antibody HPA043228

  

1.

 Acetate functions as an epigenetic metabolite to promote lipid synthesis under hypoxia
Gao X et al
Nat Commun 2016;7:11960
   Acetyl-CoA synthetase 2 promotes acetate utilization and maintains cancer cell growth under metabolic stress
Schug ZT et al
Cancer Cell 2015;27(1):57-71
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.