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ADCY8

ANTIBODY INFORMATION
 
 

Antibody HPA024291

  

Antibody CAB018652

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA024291   sc-20764  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 5   4  

References

    

Proper citation

 Atlas Antibodies Cat#HPA024291, RRID:AB_1844597   Santa Cruz Biotechnology Cat#sc-20764, RRID:AB_633654  

VALIDATION SUMMARY
 
 

Antibody HPA024291

  

Antibody CAB018652

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024291

  

Antibody CAB018652

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 5 tissues
Protein: detected in 54 cell types		   RNA: detected in 5 tissues
Protein: detected in 68 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:100  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA024291

  

Antibody CAB018652

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 140.1, 125.2   140.1, 125.2, 29  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA024291

  

Antibody CAB018652

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA024291

  

Antibody CAB018652

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 86    

Antigen sequence

 
DRRNSGATFTEGSWSPELPFDNIVGKQNTLAALTRNSINLLPNHLAQALH
VQSGPEEINKRIEHTIDLRSGDKLRREHIKPFSLMF
  

											 
																				

																		

										
											
Matching transcripts

												
																					ADCY8-001 - ENSP00000286355 [100%] 
ADCY8-002 - ENSP00000367161 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ADCY8-001
ADCY8-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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