We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm, cytosol & mitotic spindle.
Antibody dilution
1:167
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA004469
Antibody HPA073448
Antibody CAB015157
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows neuropil and neuronal positivity.
Immunohistochemical staining of human cerebral cortex shows distinct nuclear positivity in neuronal cells.
Expression
RNA: detected in 14 tissues Protein: detected in 68 cell types
RNA: detected in 14 tissues Protein: detected in 59 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:35
1:125
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Standard validation
Approved
Figure description
Cell bodies and dendrites of subsets of neurons were stained. Most abundant in olfactory bulb and hippocampus.
WESTERN BLOT
Antibody HPA004469
Antibody HPA073448
Antibody CAB015157
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
117.4, 107.3
117.4, 107.3
117.4, 107.3
Antibody dilution
1:250
1:550
1:500
PROTEIN ARRAY
Antibody HPA004469
Antibody HPA073448
Antibody CAB015157
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
