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MAPK13

ANTIBODY INFORMATION
 
 

Antibody HPA007667

  

Antibody CAB025854

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA007667   AF1519  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 3   6  

References

    

Proper citation

 Atlas Antibodies Cat#HPA007667, RRID:AB_1079867    

VALIDATION SUMMARY
 
 

Antibody HPA007667

  

Antibody CAB025854

  
Immunocytochemistry

Approved    
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA007667

  

Antibody CAB025854

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & cytosol.    

Antibody dilution

 1:83    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007667

  

Antibody CAB025854

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp and white pulp).   Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and nuclear positivity in urothelial cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 59 cell types		   RNA: detected in 35 tissues
Protein: detected in 68 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:700  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007667

  

Antibody CAB025854

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 42.1   42.1, 28.8, 17.3  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA007667

  

Antibody CAB025854

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA007667

  

Antibody CAB025854

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 138    

Antigen sequence

 
KTLFKGKDYLDQLTQILKVTGVPGTEFVQKLNDKAAKSYIQSLPQTPRKD
FTQLFPRASPQAADLLEKMLELDVDKRLTAAQALTHPFFEPFRDPEEETE
AQQPFDDSLEHEKLTVDEWKQHIYKEIVNFSPIARKDS
  

											 
																				

																		

										
											
Matching transcripts

												
																					MAPK13-001 - ENSP00000211287 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
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