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MS4A1

ANTIBODY INFORMATION
 
 

Antibody HPA014341

  

Antibody HPA014391

  

Antibody CAB000015

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
  

Product name

 HPA014341   HPA014391   M0755  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Supernatant  

Other gene match

      

Released in version

 4   4   1  

References

      

Proper citation

 Atlas Antibodies Cat#HPA014341, RRID:AB_1846265   Atlas Antibodies Cat#HPA014391, RRID:AB_1846264   Dako Cat#M0755, RRID:AB_2282030  

VALIDATION SUMMARY
 
 

Antibody HPA014341

  

Antibody HPA014391

  

Antibody CAB000015

  
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  

Validated
  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA014341

  

Antibody HPA014391

  

Antibody CAB000015

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA014391 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA014341 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells of white pulp.   Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in lymphoid cells.   Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in white pulp.  

Expression

 RNA: detected in 28 tissues
Protein: detected in 11 cell types		   RNA: detected in 28 tissues
Protein: detected in 24 cell types		   RNA: detected in 28 tissues
Protein: detected in 9 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH9  

Antibody dilution

 1:400   1:25   1:1000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA014341

  

Antibody HPA014391

  

Antibody CAB000015

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 33.1, 31.6, 14.6   33.1, 31.6   33.1, 31.6, 14.6, 13.7, 0.8, 0.7  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA014341

  

Antibody HPA014391

  

Antibody CAB000015

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA014341

  

Antibody HPA014391

  

Antibody CAB000015

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Native protein  

Length (aa)

 78   36    

Antigen sequence

 
ENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDI
EIIPIQEEEEEETETNFPEPPQDQESSP
  
MESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCY
  

											 
																				

																		

										
											
Matching transcripts

												
																					MS4A1-001 - ENSP00000433277 [100%] 
MS4A1-002 - ENSP00000433519 [100%] 
MS4A1-003 - ENSP00000432270 [100%] 
MS4A1-201 - ENSP00000314620 [100%] 
MS4A1-202 - ENSP00000374589 [100%] 

											 
																						MS4A1-001 - ENSP00000433277 [100%] 
MS4A1-002 - ENSP00000433519 [100%] 
MS4A1-201 - ENSP00000314620 [100%] 
MS4A1-202 - ENSP00000374589 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

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MS4A1-001
MS4A1-002
MS4A1-003
MS4A1-201
MS4A1-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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