Cell atlas - ATAD2 - The Human Protein Atlas

GENERAL INFORMATION
Gene name	ATAD2
Gene description	ATPase family, AAA domain containing 2
Protein class	Enzymes Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	2
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the nucleoplasm.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nucleoplasm (validated)
Single-cell variation	Single-cell variation in protein expression observed.
Cell cycle dependency	Cell cycle dependent expression determined by custom assay
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY
Reliability score	Validated based on 1 antibody. HPA029424
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the nucleoplasm.

Main location

Nucleoplasm (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location	Single-cell variation	Cell cycle dependent variation
HPA029424	A-431	Nucleoplasm	Intensity
HPA029424	U-2 OS	Nucleoplasm	Intensity	Intensity
HPA029424	U-251 MG	Nucleoplasm

CUSTOM DATA


	Toggle channels

Thumbnail	Antibody	Cell line	Assay	Description
	HPA029424	U-2 OS	Image	Staining of U-2 OS FUCCI cells, to characterize the cell cycle dependency of the protein expression pattern. The FUCCI cells, Fluorescence Ubiquitination Cell Cycle Indicator, are cells tagged with different fluorescent proteins fused to two different cell cycle regulators Cdt1 (expressed in G1 phase) and Geminin (expressed in S and G2 phases) that allows cell cycle monitoring. When both proteins are present, the overlay of the images appear in yellow marking the G1/S transition. ATAD2 is mostly expressed at the G1/S checkpoint, during S and/or G2.

CELL CYCLE
Summary	Expression has cell cycle dependency according to custom assay
Intensity variation	Nucleoplasm

siRNA VALIDATION

Antibody

HPA029424

siRNA validation

Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

Nuclear region of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

37%

19%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Sample	Cell line
HPA029424	siRNA 2	U-2 OS
HPA029424	scrambled	U-2 OS
HPA029424	siRNA 1	U-2 OS

GENE INFORMATION
Gene name	ATAD2 (HGNC Symbol)
Synonyms	CT137, DKFZp667N1320, MGC29843, MGC5254, PRO2000
Description	ATPase family, AAA domain containing 2 (HGNC Symbol)
Entrez gene summary	A large family of ATPases has been described, whose key feature is that they share a conserved region of about 220 amino acids that contains an ATP-binding site. The proteins that belong to this family either contain one or two AAA (ATPases Associated with diverse cellular Activities) domains. AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes. The protein encoded by this gene contains two AAA domains, as well as a bromodomain. [provided by RefSeq, Jul 2008]
Chromosome	8
Cytoband	q24.13
Chromosome location (bp)	123319850 - 123416350
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000156802 (version 83.38)
Entrez gene	29028
UniProt	Q6PL18 (UniProt - Evidence at protein level)
neXtProt	NX_Q6PL18
Antibodypedia	ATAD2 antibodies

PROTEIN BROWSER

ATAD2-001	ATAD2-002

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
ATAD2-001 ENSP00000287394 ENST00000287394	Q6PL18 [Direct mapping] ATPase family AAA domain-containing protein 2 A0A024R9G7 [Target identity:100%; Query identity:100%] ATPase family, AAA domain containing 2, isoform CRA_c Show all	Enzymes ENZYME proteins Hydrolases Predicted intracellular proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0003682 [chromatin binding] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0006351 [transcription, DNA-templated] GO:0016887 [ATPase activity] GO:0031936 [negative regulation of chromatin silencing] GO:0042393 [histone binding] GO:0045893 [positive regulation of transcription, DNA-templated] GO:0045944 [positive regulation of transcription from RNA polymerase II promoter] GO:0070062 [extracellular exosome] Show all	1390 aa 158.6 kDa	No	0
ATAD2-002 ENSP00000429213 ENST00000521903	A0A0B4J211 [Direct mapping] ATPase family AAA domain-containing protein 2 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0003682 [chromatin binding] GO:0005515 [protein binding] GO:0005634 [nucleus] GO:0031936 [negative regulation of chromatin silencing] GO:0042393 [histone binding] GO:0045944 [positive regulation of transcription from RNA polymerase II promoter] Show all	708 aa 80.6 kDa	No	0