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BUB1B

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GENERAL INFORMATION

Gene name

 BUB1B

Gene description

 BUB1 mitotic checkpoint serine/threonine kinase B

Protein class

 Cancer-related genes
Disease related genes
Enzymes
Potential drug targets
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 2
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HUMAN PROTEIN ATLAS INFORMATION

Summary

 Localized to the cytosol.

RNA cell category

 Expressed in all

Protein evidence

 Evidence at protein level

Main location

 Localized to the Cytosol (supported)

Single-cell variation

 Single-cell variation in protein expression observed.

Cell cycle dependency

 Cell cycle dependent expression determined by custom assay

DATA RELIABILITY

Reliability score

 Supported based on 1 antibody.
HPA008419
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ASSAYS
RNA expression
Human cells
Custom data
Mouse cells

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Localized to the cytosol.

Main location

 Cytosol (supported)

 

 

 
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Low
High
G1
S
G2
M
N/A

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Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
HPA008419 A-431 Cytosol Intensity
HPA008419 U-2 OS Cytosol Intensity Intensity
HPA008419 U-251 MG Cytosol

CUSTOM DATA

 

 

 
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Thumbnail

Antibody

Cell line

Assay

Description
HPA008419 U-2 OS Image Staining of U-2 OS FUCCI cells, to characterize the cell cycle dependency of the protein expression pattern. The FUCCI cells, Fluorescence Ubiquitination Cell Cycle Indicator, are cells tagged with different fluorescent proteins fused to two different cell cycle regulators Cdt1 (expressed in G1 phase) and Geminin (expressed in S and G2 phases) that allows cell cycle monitoring. When both proteins are present, the overlay of the images appear in yellow marking the G1/S transition. BUB1B is mostly expressed during S and/or G2, and during Mitosis.

CELL CYCLE

Summary

 Expression has cell cycle dependency according to custom assay

Intensity variation

 Cytosol

MOUSE CELLS

Main location

 Not available

Mouse gene

 ENSMUSG00000040084 (version 83)

 

 

 
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Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location
HPA008419 NIH 3T3 No staining

GENE INFORMATION

Gene name

 BUB1B (HGNC Symbol)

Synonyms

 Bub1A, BUBR1, MAD3L, SSK1

Description

 BUB1 mitotic checkpoint serine/threonine kinase B (HGNC Symbol)

Entrez gene summary

 This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008]

Chromosome

 15

Cytoband

 q15.1

Chromosome location (bp)

 40161023 - 40221136

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000156970 (version 83.38)

Entrez gene

 701

UniProt

 O60566 (UniProt - Evidence at protein level)

neXtProt

 NX_O60566

Antibodypedia

 BUB1B antibodies

PROTEIN BROWSER
BUB1B-001
BUB1B-003

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
BUB1B-001
ENSP00000287598
ENST00000287598
O60566 [Direct mapping]
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
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Enzymes
   ENZYME proteins
   Transferases
   Kinases
   MEMSAT3 predicted membrane proteins
   THUMBUP predicted membrane proteins
Predicted intracellular proteins
Cancer-related genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Nonsense Mutations
   COSMIC Missense Mutations
   COSMIC Germline Mutations
   COSMIC Frameshift Mutations
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000278 [mitotic cell cycle]
GO:0000776 [kinetochore]
GO:0000777 [condensed chromosome kinetochore]
GO:0000778 [condensed nuclear chromosome kinetochore]
GO:0000940 [condensed chromosome outer kinetochore]
GO:0004672 [protein kinase activity]
GO:0004674 [protein serine/threonine kinase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005680 [anaphase-promoting complex]
GO:0005737 [cytoplasm]
GO:0005815 [microtubule organizing center]
GO:0005829 [cytosol]
GO:0006468 [protein phosphorylation]
GO:0006915 [apoptotic process]
GO:0007067 [mitotic nuclear division]
GO:0007091 [metaphase/anaphase transition of mitotic cell cycle]
GO:0007093 [mitotic cell cycle checkpoint]
GO:0007094 [mitotic spindle assembly checkpoint]
GO:0007264 [small GTPase mediated signal transduction]
GO:0008283 [cell proliferation]
GO:0031145 [anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process]
GO:0034501 [protein localization to kinetochore]
GO:0048471 [perinuclear region of cytoplasm]
GO:0051233 [spindle midzone]
GO:0051301 [cell division]
GO:0051436 [negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle]
GO:0051439 [regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle]
GO:0071459 [protein localization to chromosome, centromeric region]
Show all
 1050 aa
119.5 kDa 		No 0
BUB1B-003
ENSP00000398470
ENST00000412359
O60566 [Direct mapping]
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
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Enzymes
   ENZYME proteins
   Transferases
   Kinases
   MEMSAT3 predicted membrane proteins
   SCAMPI predicted membrane proteins
   THUMBUP predicted membrane proteins
Predicted intracellular proteins
Cancer-related genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Nonsense Mutations
   COSMIC Missense Mutations
   COSMIC Germline Mutations
   COSMIC Frameshift Mutations
Disease related genes
Potential drug targets
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000278 [mitotic cell cycle]
GO:0000776 [kinetochore]
GO:0000777 [condensed chromosome kinetochore]
GO:0000940 [condensed chromosome outer kinetochore]
GO:0004672 [protein kinase activity]
GO:0004674 [protein serine/threonine kinase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005680 [anaphase-promoting complex]
GO:0005737 [cytoplasm]
GO:0005815 [microtubule organizing center]
GO:0005829 [cytosol]
GO:0006468 [protein phosphorylation]
GO:0006915 [apoptotic process]
GO:0007067 [mitotic nuclear division]
GO:0007093 [mitotic cell cycle checkpoint]
GO:0007094 [mitotic spindle assembly checkpoint]
GO:0007264 [small GTPase mediated signal transduction]
GO:0008283 [cell proliferation]
GO:0031145 [anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process]
GO:0034501 [protein localization to kinetochore]
GO:0048471 [perinuclear region of cytoplasm]
GO:0051233 [spindle midzone]
GO:0051301 [cell division]
GO:0051436 [negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle]
GO:0051439 [regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle]
Show all
 1064 aa
121.4 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.