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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
Antibody dilution
1:57
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA006694
Antibody HPA016950
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human lymph node shows strong nuclear positivity in germinal center cells and non-germinal center cells.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts and Leydig cells.
Expression
RNA: detected in 36 tissues Protein: detected in 70 cell types
RNA: detected in 36 tissues Protein: detected in 37 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:15
1:800
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA006694
Antibody HPA016950
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402132)
Target mass (kDa)
121.3
121.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA006694
Antibody HPA016950
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.