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MMP14

ANTIBODY INFORMATION
 
 

Antibody HPA051432

  

Antibody CAB009918

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA051432   sc-30074  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 13   3  

References

    

Proper citation

 Atlas Antibodies Cat#HPA051432, RRID:AB_2681484   Santa Cruz Biotechnology Cat#sc-30074, RRID:AB_2250767  

VALIDATION SUMMARY
 
 

Antibody HPA051432

  

Antibody CAB009918

  
Immunocytochemistry

Supported    
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051432

  

Antibody CAB009918

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line HaCaT shows localization to cytosol & intermediate filaments.    

Antibody dilution

 1:29    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051432

  

Antibody CAB009918

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human colon shows strong luminal membranous positivity in glandular cells.   Immunohistochemical staining of human urinary bladder shows positivity in stromal cells and plasma.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 40 cell types		   RNA: detected in 36 tissues
Protein: detected in 60 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:200  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051432

  

Antibody CAB009918

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 65.9   65.9, 22.3  

Antibody dilution

 1:140   1:500  

PROTEIN ARRAY
 
 

Antibody HPA051432

  

Antibody CAB009918

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1150    

ANTIGEN INFORMATION
 
 

Antibody HPA051432

  

Antibody CAB009918

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 72    

Antigen sequence

 
HWVFDEASLEPGYPKHIKELGRGLPTDKIDAALFWMPNGKTYFFRGNKYY
RFNEELRAVDSEYPKNIKVWEG
  

											 
																				

																		

										
											
Matching transcripts

												
																					MMP14-001 - ENSP00000308208 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MMP14-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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