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DPYSL5

ANTIBODY INFORMATION
 
 

Antibody HPA034544

  

Antibody HPA072387

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA034544   HPA072387  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA034544, RRID:AB_10671228   Atlas Antibodies Cat#HPA072387, RRID:AB_2686516  

VALIDATION SUMMARY
 
 

Antibody HPA034544

  

Antibody HPA072387

  
Immunocytochemistry

  Approved  
Immunohistochemistry


Validated
    
Western blot

Uncertain   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA034544

  

Antibody HPA072387

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.  

Antibody dilution

   1:9  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA034544

  

Antibody HPA072387

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human lateral ventricle shows moderate cytoplasmic positivity in neuronal cells.    

Expression

 RNA: detected in 3 tissues
Protein: detected in 8 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:900    

Literature conformity

 Consistent with gene/protein characterization data.    

RNA consistency

 Consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA034544

  

Antibody HPA072387

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 61.4   61.4  

Antibody dilution

 1:250   1:80  

PROTEIN ARRAY
 
 

Antibody HPA034544

  

Antibody HPA072387

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA034544

  

Antibody HPA072387

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 51   86  

Antigen sequence

 
GRVVYENGVFMCAEGTGKFCPLRSFPDTVYKKLVQREKTLKVRGVDRTPY
L
  
CPIYLVNVSSISAGDVIAAAKMQGKVVLAETTTAHATLTGLHYYHQDWSH
AAAYVTVPPLRLDTNTSTYLMSLLANDTLNIVASDH
 

Matching transcripts

 DPYSL5-001 - ENSP00000288699 [100%]
DPYSL5-002 - ENSP00000385549 [100%]
DPYSL5-201 - ENSP00000481305 [100%]
  DPYSL5-001 - ENSP00000288699 [100%]
DPYSL5-002 - ENSP00000385549 [100%]
DPYSL5-201 - ENSP00000481305 [100%]
 

Other gene match

    
ANTIGEN VIEW
DPYSL5-001
DPYSL5-002
DPYSL5-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.