Fields »
ABOUT
HELP
BLOG
FANCC

ANTIBODY INFORMATION
 
 

Antibody HPA030771

  

Antibody CAB017793

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA030771   2529.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 14   4  

References

    

Proper citation

 Atlas Antibodies Cat#HPA030771, RRID:AB_2673604    

VALIDATION SUMMARY
 
 

Antibody HPA030771

  

Antibody CAB017793

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Approved  
Western blot

Supported   Uncertain  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA030771

  

Antibody CAB017793

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.    

Antibody dilution

 1:37    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA030771

  

Antibody CAB017793

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human rectum shows nuclear and cytoplasmic positivity in glandular cells.  

Expression

   RNA: detected in 36 tissues
Protein: detected in 68 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:90  

Literature conformity

   Consistent with gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA030771

  

Antibody CAB017793

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 63.4, 56.1   63.4, 56.1, 17.3  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA030771

  

Antibody CAB017793

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA030771

  

Antibody CAB017793

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 120    

Antigen sequence

 
NSGQSKLNSWIQGVLSHILSALRFDKEVALFTQGLGYAPIDYYPGLLKNM
VLSLASELRENHLNGFNTQRRMAPERVASLSRVCVPLITLTDVDPLVEAL
LICHGREPQEILQPEFFEAV
  

											 
																				

																		

										
											
Matching transcripts

												
																					FANCC-001 - ENSP00000289081 [100%] 
FANCC-002 - ENSP00000364454 [100%] 
FANCC-201 - ENSP00000479931 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
FANCC-001
FANCC-002
FANCC-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.