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ANTIBODY INFORMATION
 
 

Antibody HPA001931

  

Antibody CAB026191

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA001931   MAB4357  

Host species

 Rabbit   Rat  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 3   6  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA001931, RRID:AB_1079620    

VALIDATION SUMMARY
 
 

Antibody HPA001931

  

Antibody CAB026191

  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001931

  

Antibody CAB026191

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 55 cell types		   RNA: detected in 37 tissues
Protein: detected in 77 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:200  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001931

  

Antibody CAB026191

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 62.8   62.8  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001931

  

Antibody CAB026191

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA001931

  

Antibody CAB026191

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 124    

Antigen sequence

 
GVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQL
PFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFG
LVNPFYGQGKAHLESRSYQEAQLP
  

											 
																				

																		

										
											
Matching transcripts

												
																					PINK1-001 - ENSP00000364204 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PINK1-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001931

												
										 
																				
											
											
											
Antibody CAB026191

												
										 
																		

																	

										
											
1.

												
																						BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria

	Choubey V et al

	Autophagy 2014;10(6):1105-19

			Application: IP				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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