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ANTIBODY INFORMATION
 
 

Antibody HPA023535

  

Antibody HPA031755

  

Antibody HPA031756

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA023535   HPA031755   HPA031756  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 5   12   12  

References

 3      

Proper citation

 Atlas Antibodies Cat#HPA023535, RRID:AB_1857362   Atlas Antibodies Cat#HPA031755, RRID:AB_2674029   Atlas Antibodies Cat#HPA031756, RRID:AB_2674030  

VALIDATION SUMMARY
 
 

Antibody HPA023535

  

Antibody HPA031755

  

Antibody HPA031756

  
Immunocytochemistry


Validated
  

Validated
  

Validated
  
Immunohistochemistry

Supported      
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA023535

  

Antibody HPA031755

  

Antibody HPA031756

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA031756.
Antibody staining overlaps with antibody HPA031755.		  
Validated

Antibody staining overlaps with antibody HPA031756.
Antibody staining overlaps with antibody HPA023535.		  
Validated

Antibody staining overlaps with antibody HPA031755.
Antibody staining overlaps with antibody HPA023535.		  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.   Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.   Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.  

Antibody dilution

 1:50   1:200   1:6  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA023535

  

Antibody HPA031755

  

Antibody HPA031756

  

Standard validation

 Supported      

     

Figure description

 Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.      

Expression

 RNA: detected in 37 tissues
Protein: detected in 74 cell types		      

Retrieval

 HIER pH6      

Antibody dilution

 1:750      

Literature conformity

 Consistent with extensive gene/protein characterization data.      

RNA consistency

 Mainly consistent with RNA expression data.      

WESTERN BLOT
 
 

Antibody HPA023535

  

Antibody HPA031755

  

Antibody HPA031756

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 263.8, 250.4, 228.2, 159.9   263.8, 250.4, 228.2, 159.9   263.8, 250.4, 228.2  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA023535

  

Antibody HPA031755

  

Antibody HPA031756

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA023535

  

Antibody HPA031755

  

Antibody HPA031756

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 136   95   77  

Antigen sequence

 
TEVAIESTPMILESSIMSSHVMKGINLSSGDQNLAPEIGMQEIALHSGEE
PHAEEHLKGDFYESEHGINIDLNINNHLIAKEMEHNTVCAAGTSPVGEIG
EEKILPTSETKQRTVLDTYPGVSEADAGETLSSTGP
  
TCMVSETPAMSAEPTVLASEPPVMSETAETFDSMRASGHVASEVSTSLLV
PAVTTPVLAESILEPPAMAAPESSAMAVLESSAVTVLESSTVTVL
  
PVAKVLEPSETLVVSSETPTEVYPEPSTSTTMDFPESSAIEALRLPEQPV
DVPSEIADSSMTRPQELPELPKTTALE
 

Matching transcripts

 SON-001 - ENSP00000348984 [100%]
SON-003 - ENSP00000300278 [100%]
SON-006 - ENSP00000371095 [100%]
SON-007 - ENSP00000400985 [100%]
  SON-001 - ENSP00000348984 [100%]
SON-003 - ENSP00000300278 [100%]
SON-006 - ENSP00000371095 [100%]
SON-007 - ENSP00000400985 [100%]
  SON-001 - ENSP00000348984 [100%]
SON-003 - ENSP00000300278 [100%]
SON-006 - ENSP00000371095 [100%]
 

Other gene match

      
ANTIGEN VIEW
SON-001
SON-003
SON-006
SON-007

RELEVANT PUBLICATIONS
 
 

Antibody HPA023535

  

Antibody HPA031755

  

Antibody HPA031756

  

1.

 Clustering phenotype populations by genome-wide RNAi and multiparametric imaging
Fuchs F et al
Mol Syst Biol 2010;6:370
      

2.

 Antibody-based protein profiling of the human chromosome 21
Uhlén M et al
Mol Cell Proteomics 2012;11(3):M111.013458
      

3.

 The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in human cells
Ammon T et al
J Mol Cell Biol 2014;6(4):312-23
      
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.