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ATP13A2

ANTIBODY INFORMATION
 
 

Antibody HPA050910

  

Antibody HPA054717

  

Antibody CAB037038

  

Antibody CAB037111

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SIGMA
   SIGMA
  

Product name

 HPA050910   HPA054717   A9732   A9607  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity   Affinity  

Other gene match

        

Released in version

 14   14   14   7  

References

        

Proper citation

 Atlas Antibodies Cat#HPA050910, RRID:AB_2681269   Atlas Antibodies Cat#HPA054717, RRID:AB_2682583   Sigma-Aldrich Cat#A9732, RRID:AB_1840715   Sigma-Aldrich Cat#A9607, RRID:AB_1840714  

VALIDATION SUMMARY
 
 

Antibody HPA050910

  

Antibody HPA054717

  

Antibody CAB037038

  

Antibody CAB037111

  
Immunohistochemistry

Approved   Approved   Approved   Approved  
Western blot

Supported   Supported   Uncertain   Supported  
Protein array

Supported   Supported      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA050910

  

Antibody HPA054717

  

Antibody CAB037038

  

Antibody CAB037111

  

Standard validation

 Approved   Approved   Approved   Approved  

       

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells and islets of Langerhans.   Immunohistochemical staining of human nasopharynx shows strong cytoplasmic and nuclear positivity in respiratory epithelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 64 cell types		   RNA: detected in 37 tissues
Protein: detected in 56 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		   RNA: detected in 37 tissues
Protein: detected in 81 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:700   1:600   1:800  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA050910

  

Antibody HPA054717

  

Antibody CAB037038

  

Antibody CAB037111

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 128.8, 128.3   128.8, 128.3, 126, 36, 21.6, 21.5, 21.3   128.8, 128.3, 126, 42.6, 36, 28.1, 24.5, 21.6, 21.5, 21.3, 20.7   128.8, 128.3, 126, 42.6, 36, 28.1, 24.5, 21.6, 21.5, 21.3, 20.7  

Antibody dilution

 1:1050   1:350   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA050910

  

Antibody HPA054717

  

Antibody CAB037038

  

Antibody CAB037111

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:8400   1:2850      

ANTIGEN INFORMATION
 
 

Antibody HPA050910

  

Antibody HPA054717

  

Antibody CAB037038

  

Antibody CAB037111

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide   Synthetic peptide  

Length (aa)

 83   88      

Antigen sequence

 
RGCGMVAPQEHLIIVHATHPERGQPASLEFLPMESPTAVNGVKDPDQAAS
YTVEPDPRSRHLALSGPTFGIIVKHFPKLLPKV
  
EIRDKEDSSWQLFTVQVQTEAIGEGSLEPSPQSQAEDGRSQAAVGAVPEG
AWKDTAQLHKSEEAVSVGQKRVLRYYLFQGQRYIWIET
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					ATP13A2-001 - ENSP00000327214 [100%] 
ATP13A2-005 - ENSP00000413307 [100%] 

											 
																						ATP13A2-001 - ENSP00000327214 [100%] 
ATP13A2-009 - ENSP00000424313 [99%] 
ATP13A2-002 - ENSP00000341115 [94%] 
ATP13A2-005 - ENSP00000413307 [94%] 
ATP13A2-008 - ENSP00000422227 [94%] 
ATP13A2-010 - ENSP00000422668 [93%] 
ATP13A2-011 - ENSP00000427241 [93%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ATP13A2-001
ATP13A2-002
ATP13A2-005
ATP13A2-008
ATP13A2-009
ATP13A2-010
ATP13A2-011
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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