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Immunofluorescent staining of human cell line RT4 shows localization to nucleoli.
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Antibody dilution
1:61
1:22
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA045420
Antibody HPA059302
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Expression
RNA: detected in 8 tissues Protein: detected in 56 cell types
Retrieval
HIER pH6
Antibody dilution
1:200
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA045420
Antibody HPA059302
Standard validation
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408490)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
59.4, 58.9
59.4, 58.9, 16
Antibody dilution
1:250
1:80
PROTEIN ARRAY
Antibody HPA045420
Antibody HPA059302
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:600
ANTIGEN INFORMATION
Antibody HPA045420
Antibody HPA059302
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
102
97
Antigen sequence
RMDDPKGDFITLYQMASQSSASHYKLQVIKALKSSGLCESLTYGLPFILR
PTSCWQLDWDELETNQQHFHALCHSLLKREWLLLAKGEPPGPGHSQRIPA
ST
