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ADGRG5

ANTIBODY INFORMATION
 
 

Antibody HPA007133

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA007133  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 4  

References

  

Proper citation

 Atlas Antibodies Cat#HPA007133, RRID:AB_1849253  

VALIDATION SUMMARY
 
 

Antibody HPA007133

  
Immunocytochemistry

Approved  
Immunohistochemistry

Supported  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA007133

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm, nuclear membrane & vesicles.  

Antibody dilution

 1:10  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007133

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.  

Expression

 RNA: detected in 10 tissues
Protein: detected in 15 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:250  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007133

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 59, 24.8  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA007133

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA007133

  

Antigen

 Recombinant protein fragment  

Length (aa)

 149  

Antigen sequence

 
ETWEELLSYMENMQVSRGRSSVFSSRQLHQLEQMLLNTSFPGYNLTLQTP
TIQSLAFKLSCDFSGLSLTSATLKRVPQAGGQHARGQHAMQFPAELTRDA
CKTRPRELRLICIYFSNTHFFKDENNSSLLNNYVLGAQLSHGHVNNLRD
 

Matching transcripts

 ADGRG5-001 - ENSP00000342981 [100%]
ADGRG5-006 - ENSP00000290823 [100%]
ADGRG5-009 - ENSP00000482481 [100%]
 

Other gene match

  
ANTIGEN VIEW
ADGRG5-001
ADGRG5-006
ADGRG5-009
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.