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Immunofluorescent staining of human cell line U-2 OS shows localization to focal adhesion sites.
Antibody dilution
1:12
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA004835
Antibody HPA073497
Antibody CAB009321
Antibody CAB075747
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA073497 across 75 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA004835 across 75 cell types.
Standard validation
Approved
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows strong positivity in subset of cells in red and white pulp.
Immunohistochemical staining of human bone marrow shows distinct cytoplasmic positivity in subsets of hematopoietic cells.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
Expression
RNA: detected in 37 tissues Protein: detected in 14 cell types
RNA: detected in 37 tissues Protein: detected in 15 cell types
RNA: detected in 37 tissues Protein: detected in 51 cell types
RNA: detected in 37 tissues Protein: detected in 15 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:50
1:600
1:15
1:12000
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA004835
Antibody HPA073497
Antibody CAB009321
Antibody CAB075747
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
61.3, 57.6, 45.1
61.3, 57.6, 17.1
61.3, 57.6, 45.1, 19.1, 18.6, 17.1
61.3, 57.6, 45.1, 19.1, 18.6, 17.1
Antibody dilution
1:250
1:130
1:500
1:500
PROTEIN ARRAY
Antibody HPA004835
Antibody HPA073497
Antibody CAB009321
Antibody CAB075747
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
