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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & focal adhesion sites.
Antibody dilution
1:52
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA031768
Antibody HPA031769
Antibody HPA031770
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human placenta shows strong cytoplasmic and nucleolar positivity in decidual cells.
Immunohistochemical staining of human testis shows moderate cytoplasmic and membranous positivity in cells in seminiferus ducts with distinct staining of acrosomal caps.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
Expression
RNA: detected in 34 tissues Protein: detected in 71 cell types
RNA: detected in 34 tissues Protein: detected in 78 cell types
RNA: detected in 34 tissues Protein: detected in 48 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:300
1:250
1:75
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031768
Antibody HPA031769
Antibody HPA031770
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
50.2, 38.5
50.2, 38.5
50.2
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA031768
Antibody HPA031769
Antibody HPA031770
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
