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ATAD3B

ANTIBODY INFORMATION
 
 

Antibody HPA058968

  

Antibody HPA064867

  

Antibody HPA065305

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA058968   HPA064867   HPA065305  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 ATAD3A - ENSG00000197785 [97%]
   ATAD3A - ENSG00000197785 [100%]
   ATAD3A - ENSG00000197785 [100%]
ATAD3C - ENSG00000215915 [100%]
  

Released in version

 12   16   16  

References

      

Proper citation

 Atlas Antibodies Cat#HPA058968, RRID:AB_2683864   Atlas Antibodies Cat#HPA064867, RRID:AB_2685375   Atlas Antibodies Cat#HPA065305, RRID:AB_2685459  

VALIDATION SUMMARY
 
 

Antibody HPA058968

  

Antibody HPA064867

  

Antibody HPA065305

  
Immunocytochemistry

  Supported   Supported  
Immunohistochemistry

Approved      
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA058968

  

Antibody HPA064867

  

Antibody HPA065305

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

   1:200   1:42  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA058968

  

Antibody HPA064867

  

Antibody HPA065305

  

Standard validation

 Approved      

     

Figure description

 Immunohistochemical staining of human colon shows strong granular cytoplasmic positivity in glandular cells.      

Expression

 RNA: detected in 28 tissues
Protein: detected in 77 cell types		      

Retrieval

 HIER pH6      

Antibody dilution

 1:40      

Literature conformity

 Consistent with extensive gene/protein characterization data.      

RNA consistency

 Mainly consistent with RNA expression data.      

WESTERN BLOT
 
 

Antibody HPA058968

  

Antibody HPA064867

  

Antibody HPA065305

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 72.6, 71.4, 66.2, 64.2, 57.9   72.6, 71.4, 66.2, 64.2, 57.9   72.6, 71.4, 66.2, 64.2, 57.9, 46.4, 22.9  

Antibody dilution

 1:130   1:1420   1:140  

PROTEIN ARRAY
 
 

Antibody HPA058968

  

Antibody HPA064867

  

Antibody HPA065305

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1550   1:17050   1:1650  

ANTIGEN INFORMATION
 
 

Antibody HPA058968

  

Antibody HPA064867

  

Antibody HPA065305

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 32   68   41  

Antigen sequence

 
KAERENADIIREQIRLKASEHRQTVLESIRTA
  
EYEAAVEQLKSEQIRAQAEERRKTLSEETRQHQARAQYQDKLARQRYEDQ
LKQQQLLNEENLRKQEES
  
KLALHSGMDYAIMTGGDVAPMGREGVTAMHKLFDWANTSRR
 

Matching transcripts

 ATAD3B-001 - ENSP00000311766 [100%]
  ATAD3B-001 - ENSP00000311766 [100%]
  ATAD3B-001 - ENSP00000311766 [100%]
 

Other gene match

 ATAD3A - ENSG00000197785 [97%]
   ATAD3A - ENSG00000197785 [100%]
   ATAD3A - ENSG00000197785 [100%]
ATAD3C - ENSG00000215915 [100%]
  
ANTIGEN VIEW
ATAD3B-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.