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RRP1B

ANTIBODY INFORMATION
 
 

Antibody HPA017893

  

Antibody HPA020324

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA017893   HPA020324  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   5  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA017893, RRID:AB_1852274   Atlas Antibodies Cat#HPA020324, RRID:AB_1852276  

VALIDATION SUMMARY
 
 

Antibody HPA017893

  

Antibody HPA020324

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Supported   Supported  
Western blot


Validated
   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA017893

  

Antibody HPA020324

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA020324.		  
Validated

Antibody staining overlaps with antibody HPA017893.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.  

Antibody dilution

 1:200   1:64  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA017893

  

Antibody HPA020324

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 71 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:650   1:90  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA017893

  

Antibody HPA020324

  

Formal validation: Genetic
Validated

Downregulation visible in one of two siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 84.4    

Loading control

 Total protein image    

Antibody dilution

 1:575    
 

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 84.4   84.4  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA017893

  

Antibody HPA020324

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA017893

  

Antibody HPA020324

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 117   136  

Antigen sequence

 
SISQLSFAEDISADEDDQILSQGKHKKKGNKLLEKTNLEKEKGSRVFCVE
EEDSESSLQKRRRKKKKKHHLQPENPGPGGAAPSLEQNRGREPEASGLKA
LKARVAEPGAEATSSTG
  
VLESEAGQPQALGSSGTCSSLKKQKLRAESDFVKFDTPFLPKPLFFRRAK
SSTATHPPGPAVQLNKTPSSSKKVTFGLNRNMTAEFKKTDKSILVSPTGP
SRVAFDPEQKPLHGVLKTPTSSPASSPLVAKKPLTT
 

Matching transcripts

 RRP1B-001 - ENSP00000339145 [100%]
  RRP1B-001 - ENSP00000339145 [100%]
 

Other gene match

    
ANTIGEN VIEW
RRP1B-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA017893

  

Antibody HPA020324

  

1.

 BRD4 short isoform interacts with RRP1B, SIPA1 and components of the LINC complex at the inner face of the nuclear membrane
Alsarraj J et al
PLoS One 2013;8(11):e80746
    

2.

 RRP1B is a metastasis modifier that regulates the expression of alternative mRNA isoforms through interactions with SRSF1
Lee M et al
Oncogene 2014;33(14):1818-27
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.