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ANTIBODY INFORMATION
 
 

Antibody HPA016820

  

Antibody HPA019887

  

Antibody HPA032101

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA016820   HPA019887   HPA032101  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 5   5   12  

References

 2      

Proper citation

 Atlas Antibodies Cat#HPA016820, RRID:AB_1855079   Atlas Antibodies Cat#HPA019887, RRID:AB_1855080   Atlas Antibodies Cat#HPA032101, RRID:AB_2674151  

VALIDATION SUMMARY
 
 

Antibody HPA016820

  

Antibody HPA019887

  

Antibody HPA032101

  
Immunocytochemistry


Validated
   Supported  

Validated
  
Immunohistochemistry

Supported   Supported    
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA016820

  

Antibody HPA019887

  

Antibody HPA032101

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA032101.		    
Validated

Antibody staining overlaps with antibody HPA016820.		  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to centrosome.   Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.   Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.  

Antibody dilution

 1:196   1:75   1:200  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA016820

  

Antibody HPA019887

  

Antibody HPA032101

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in seminiferus duct cells.    

Expression

 RNA: detected in 36 tissues
Protein: detected in 61 cell types		   RNA: detected in 36 tissues
Protein: detected in 52 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:300   1:200    

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.    

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA016820

  

Antibody HPA019887

  

Antibody HPA032101

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

     

Figure description

      

Target mass (kDa)

 378   378   378  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA016820

  

Antibody HPA019887

  

Antibody HPA032101

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA016820

  

Antibody HPA019887

  

Antibody HPA032101

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 145   129   93  

Antigen sequence

 
MEDLQNQFQKELAEQRAELEKIFQDKNQAERALRNLESHHQAAIEKLRED
LQSEHGRCLEDLEFKFKESEKEKQLELENLQASYEDLKAQSQEEIRRLWS
QLDSARTSRQELSELHEQLLARTSRVEDLEQLKQREKTQHESELE
  
MLDLSSWSSPEVLRKDWTLEPWPSLPVTPHSGALSLCSADTSLGDRADTS
LPQTQGPGLLCSPGVSAAALALQWAESPPADDHHVQRTAVEKDVEDFITT
SFDSQETLSSPPPGLEGKADRSEKSDGSG
  
RLLGLFGETLRAAVTLRSRIGERVGLCLDDAGAGLALSTAPALEETWSDV
ALPELDRTLSECAEMSSVAEISSHMRESFLMSPESVRECEQPI
 

Matching transcripts

 PCNT-001 - ENSP00000352572 [100%]
  PCNT-001 - ENSP00000352572 [100%]
  PCNT-001 - ENSP00000352572 [100%]
 

Other gene match

      
ANTIGEN VIEW
PCNT-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA016820

  

Antibody HPA019887

  

Antibody HPA032101

  

1.

 Antibody-based protein profiling of the human chromosome 21
Uhlén M et al
Mol Cell Proteomics 2012;11(3):M111.013458
      

2.

 Differing effects of herpes simplex virus 1 and pseudorabies virus infections on centrosomal function
Pasdeloup D et al
J Virol 2013;87(12):7102-12
      
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.